159 Current Practice of Intra-Operative Sampling During Revision Surgery of the Knee for Prosthetic Joint Infection at a High-Volume District General Hospital

Abstract Aim Successful treatment of prosthetic joint infection (PJI) involves surgical interventions and identification infecting organisms to enable targeted antimicrobial therapy. Current PHE guidelines recommend 3–5 intra-operative samples be taken using separate instruments for each, swabs are discouraged. We audited the revision arthroplasty practice at Barking, Havering, Redbridge University Trust (BHRUT), specifically comparing sampling infected knee procedures with these guidelines. Method Retrospective cohort study all cases, as defined by Musculoskeletal Infection Society criteria, BHRUT between December 2011 May 2020. A review case notes collected various key data points, focusing on intraoperative sample, type, number, technique. Point biserial correlation analysis was performed determine statistical significance. Results 119 were in 8.5-year period. 31 (26%) (n=29 patients), n=2 patients had re-revision second PJI. Number ranged from 1–12 a mode 6. N=27 (87%) cases recommended number total taken, n= 20 (65%) five or more N=24 (77%) three n=18 (58%) samples. Conclusions management presents great variability Sample collection adheres national 77% cases. implementation standardised approach through inclusion scalpel forceps pack theatre suspected PJI's.